Thermo Scientific NanoDrop 8000
Thermo Scientific NanoDrop 8000

Thermo

Thermo Scientific NanoDrop 8000 UV-Vis Spectrophotometers are used in academic, biopharmaceutical, core and bioproduction facilities, to measure between 1 and 8 microvolume nucleic acid and protein samples simultaneously.  The patented sample retention system allows you to assess the concentration and purity of samples as small as 1µL, while the multi sample capability allows you to analyze samples more efficiently.

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  • Wide spectral range (190-840nm) for measuring a variety of samples types:

– Peptides (205nm)
– DNA and RNA (260nm)
– Purified protein (280nm)
– Toxicology assays and industrial dyes (490nm)
– Gold nanoparticles (520nm)
– Colorimetric protein assay (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
– Optical Density measurements (600 nm)

  • No dilutions required
  • Only requires 1-2 µL of your sample
  • Calculates sample purity ratios (260/280 nm and 260/230 nm)
  • Measure up to 96 samples in less than six minutes
  • Choose to measure form 1 to 8 samples in one measurement cycle
  • Pre-configured methods for DNA, Protein A280, Microarray, Protein and Labels, Pierce 660, Bradford, BCA, and Lowry
  • Patented sample retention system automatically optimizes pathlength to accommodate low and high concentrations (2.5 to 3,700 ng/µL dsDNA)
  • User-friendly software that includes custom methods and data export capabilities
NanoDrop 8000
Instrument Type   Spectrophotometer
Sample size 1 µL
Sample Number up to 8
Path lengths 1 mm and 0.2 mm
Light Source Xenon flash lamp
Detector Type 2048-element linear silicon CCD
Wavelength range 220-750 nm
Wavelength accuracy 1 nm
Wavelength resolution 3 nm (FWHM at Hg 546 nm)
Recommended Sample Volume, Nucleic acid 1 µL
Recommended Sample Volume, Proteins 2 µL
Absorbance  Precision 0.003 absorbance (1 mm path)
Absorbance Accuracy 3% (at .74 absorbance at 350 nm)
Absorbance Range 0.05-75 (10 mm equivalent absorbance)
Detection Limit 2.5 ng/µL (dsDNA)
Maximum Concentration 3700 ng/µL (dsDNA)
Measurement Cycle Time 20 seconds (for 8 samples)
Dimensions (footprint) 24 cm X 32 cm
Weight 3.4 kg
Sample Pedestal Material of Construction 303 stainless steel and quartz fiber
Operating voltage 12 vdc
Operating Power Consumption 30 W
Standby Power Consumption 6 W
Software Compatibility Professional versions of Windows 7 (32 bit and 64 bit), Windows Vista (32 bit), Windows XP (32 bit)
UL/CSA and CE approval All units
Recommended sample volume, Nucleic acid 1uL
Recommended sample volume, Proteins 2uL
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